VDL Frozen Semen Thawing Instructions
Water Bath (max 2 liter) with wide neck (Temp = 37 degree C)
Pair of scissors
Pair of fine pliers
Liquid nitrogen container, containing the dose of semen. Note
that each collection dose has a different number of straws per
insemination. Your shipment should have come with paperwork
that tells you the number of straws for you to use for each
dose. If you do not have this, contact us or VDL Stud.
15 ml tubes
20 ml syringes
Clean Paper Towels
Have the mare ready and stand-by for insemination.
Place the liquid nitrogen container as close as possible to the Water
Bath. Place thermometer into the Water Bath and make sure
temperature is at 37 degrees C.
Take one straw out of the visotube with a pair of pliers and put it
quickly (with the sealed side up)into the 37 degree C. Water Bath.
Put the straws one at a time into the Water Bath. Do not put
the straws in at the same time.
Start the stopwatch after the last straw. After 30 seconds,
take the straws out and dry them off with a clean paper towel.
Cut the sealed side and put the straw upside down in a 15 ml
pre-warmed tube (37 degree C). Cut the other size off and the
straw will empty itself inside the pre-warmed tube.
Empty the other straws the same way.
Fill the insemination pepette by introducing the pipette into the
pre-warmed tube and sucking the semen into the pipette.
Inseminate the mare immediately. Be sure to inseminate your
mare on the right moment! We recommend within 6 hours of post
ovulation, but the closer to ovulation, the better chane you ahve for
WARNING: Do not keep the frozen semen canister in the nitrogen
tank longer than 8 seconds above the liquid nitrogen or the semen
will be damaged. Have all your materials very clean, better sterilized.
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